pGlo Lab Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB 0 0 0 - pGLO LB/amp 0 0 0 + pGLO LB/amp 0 0 0 + pGLO LB/amp/ara 6 6 6

2.	What two new traits do your transformed bacteria have?
	The transformed bacteria now glow green in ultraviolet light, and are immune to ampicillin, an antibiotic.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	I think that the bacteria multiplied to 600uL at least, being that there were six colonies coming from one.
4.	What is the role of arabinose in the plates?
	To give the bacteria energy in order to glow.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	-Vector for genetically modified organisms. -Used to make organisms fluorescent. -Used to make organisms ampicillin resistant.
6.	Give an example of another application of genetic engineering.

                 Genetically making an identical copy of an organism; cloning.